eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Mounting

SEM

  1. Paint carbon onto stub.
  2. Drop pieces into paint, and let dry.
  3. Coat with 5 nm gold for FESEM.

TEM

  1. Embed sample in epoxy.
  2. Cut with ultramicrotome.
  3. Mount on grid and view in TEM.

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