eTraining Introduction

Specimen Preparation

Leica Ultracut UCT



Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB


Veeco Dim 3000 AFM

Fluorescence Microscopes


Safety Procedures


Initial Settings

FIB Initial SettingsWhen you begin a session on the FIB, do not assume that all of the controls are properly set. Prior to starting the session, the IP1, IP2, and DP/Water/Air Press lights should be all be green. In addition, the S.C. and S.E.C. VACUUM lights should be lit green and on HIGH. Always check the conditions before you begin using the Brief Operating Instruction sheet found in the lab.

Specimen Height

The size of a specimen is constrained by the dimension of the goniometer stage. Specimens should fit the dimensions of 5x8x1 mm. If the specimen is too large in width, it will not enter the goniometer stage properly and could become trapped. If the specimen is too large in height, there is a risk of the deposition gun nozzle striking the specimen. Moreover, exceeding the normal height dimension could make it difficult to bring the specimen into focus.

Exchange Rod

The specimen holder is inserted into the specimen chamber through the goniometer stage. NOTE: the grease used to lubricate the rod is toxic; therefore, DO NOT TOUCH THE EXCHANGE ROD ITSELF!


When inserting or removing the specimen holder rod from the goniometer stage, insert the holder into the stage in a straight line. Any vertical or horizontal torque can flex the o-ring on the specimen holder, momentarily breaking the seal. Vacuum leaks can be avoided by applying little or no torque to the rod. If the chamber vacuum is lost, call lab personnel.

Electrical and General Safety

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