eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Specimen Exchange

NOTE: ALWAYS USE GLOVES WHEN HANDLING SPECIMENS AND HOLDERS!

Specimen Exchange

1. Always check the specimen height using the height gauge. VIEW
2. Turn on the chamber camera and monitor. VIEW
3. Ensure that the vacuum readings are within range. Make sure the "SEC" switch position is selected on the front panel, and hit the AIR button to vent the exchange chamber. VIEW
4. Pull the door open by grabbing the door itself, not the rod. VIEW
5. Screw the specimen holder onto the end of the rod. VIEW
6. Pull the rod back into the locked position and close the door.    
7. Press the EVAC button to evacuate the exchange chamber. VIEW
8. Wait until the SEC vacuum reads "high" on the front panel. VIEW
9. Turn the handle counterclockwise to open MV1. VIEW
10. While observing the chamber camera, push the rod onto the dovetail on the stage and unscrew the rod.    
11. Pull the rod out until it locks and close MV1. You do not need to AIR the chamber!    

Top of Page