eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Microanalysis

X-Ray Mapping

NOTE: The current drops quickly over the first 30 minutes of use. Wait until the current is stable before X-ray mapping.

1. Select Mode > EDS Image. VIEW
Setup Icon2. Select the Setup icon. Choose an appropriate dwell time in EDX Image Acquisition.
NOTE: For the low resolution test collection, use a dwell of 5 ms. For any higher resolution maps, a dwell time between 5 and 20 ms is suitable.
In the EDX Image Setup window, choose Edit Setup under EDX Image Processing.
VIEW
3. Select elements by clicking in the element's box. VIEW
4. To assign a different color to each element, right-click on the element > select Edit > Set Color > and choose a color. Click OK. VIEW
5. Select OK in the EDX Image Setup menu once all of your choices have been made.    
6. Choose the correct magnification and kV in Options. NOTE: If the correct magnification is not in the preset menu, you will be able to manually enter the correct magnification in the Step 7 after selecting the camera icon. VIEW
7. Perform a quick acquisition first by choosing a low resolution map of 320x240 in EDX Image. VIEW
Camera Icon8. Select the camera icon in EDX Image. Once you select the icon, a magnification dialog box appears. Enter the correct magnification. The map consists of a secondary electron image and a scan for each element chosen. VIEW
9. Once the acquisition has begun, you can still edit the collection in live time by right-clicking in the image area and selecting ROI Processing. A menu with the same functions as the Setup menu will appear. Use the directions in Step 3 to edit. VIEW
10. If the map is satisfactory, perform again using a high resolution.    
11. Save the map in your directory in the “Temporary User Files” folder.    

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