eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Microanalysis

EDS X-Ray Mapping

1. Collect a spectrum and choose the elements to map.    
2. Select Mode > EDS Image. VIEW
Setup Icon3. Select the Setup icon and choose an appropriate dwell time in EDX Image Setup. Choose Slow Map, Frames = 1 and check the Line Sync box. Enable Channel 1 to add an image of the specimen to your dataset. The image will be SE or BSE depending on the microscope setting. NOTE: For the low resolution test collection, use a dwell time of 5 ms. For any higher resolution maps, a dwell time between 5 and 20 ms is suitable. VIEW
4. In the EDX Image Setup menu, select Manual ROI and choose Edit Setup. Select the elements you want by clicking in the element's box. VIEW
5. To assign a different color to each element, right-click on the element > select Edit > Set Color > and choose a color. Click OK. VIEW
6. Select OK in the EDX Image Setup menu once all of your choices have been made.    
7. Choose the correct magnification and kV in Options. NOTE: If the correct magnification is not in the preset menu, you can select Ask & Add which will prompt you to enter the magnification. VIEW
8. Adjust the Dead Time to be approximately 45% by using the Probe Current Knob on the Main Control Panel. It can be further adjusted by using the Fine Condenser Lens setting in the EOS Menu, Use the arrow keys to highlight the Fine Condenser lens and then use +/- buttons to adjust.    
9. Perform a quick acquisition first by choosing a low resolution map of 128x128 in EDX Image. VIEW
10. Once the acquisition has begun, you can still edit the collection in live time by right clicking in the image area and selecting ROI Processing. VIEW
11. If the test map is satisfactory, perform a new map again at higher resolution using the procedure described above.    
12. Right click in the dataset window and choose Extract all Save the map in your directory in the "User files" folder.    

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