eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Fluorescence Microscopy

AxioVision Toolbar

AxioVision Toolbar

The software and icons refer to the AxioVision software for Windows. Click the toolbar above for a larger view.

1. Select the correct filter for the observed fluorescence by rotating the filter turret using the forward and backward REFLECTOR keys.

The filter set currently in use will be listed in the lower left of the LCD display on the top of the microscope.
VIEW
2. Open the fluorescence shutter by depressing the FL on/off key.

NOTE: When not observing fluorescence, close the fluorescence shutter to minimize sample fluorescence decay.
VIEW
3. When observing a single fluorophore:    
 Live Icon3.1. Use the AxioCamMR: Live screen to adjust the exposure and focus of the image in the same way as for transmitted light. VIEW
 Snap Icon3.2. Capture the image by selecting the Snap icon.    
 3.3. Add color to the image by maximizing the captured image window. In the lower left, there will be several options for image modification. Right click on the number icon and select the correct color. Then, click on the color wheel icon so that it reads “On”. VIEW
6D Acquisition Icon4. For observing multiple fluorophores, select the 6-D Acquisition tab.    
 6D Color Icon4.1. Under the C tab, select colors by right clicking to eliminate and turn on channels. VIEW
 4.2. Select one of the color channels by left clicking, then select measure. In the new window, adjust the exposure in the same way as for a regular image and then select OK. See Basic Operation under Zeiss AxioVert 200 M and ApoTome in Fluorescence Microscopes.    
 4.3. Repeat step 4.2. for each channel and then select Start.    

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