eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Basic Operation

This section describes viewing in transmitted light.

DP Controller Main Window

DP Main Window

The software and icons refer to the DP Controller software for Windows. DP refers to the Olympus DP70 Digital Microscope Camera. Click the toolbars above to view the main window.

NOTE: The Olympus BX51 is only capable of brightfield transmitted light imaging. Therefore, the specimens which can be imaged using transmitted light are limited to stained or colored samples mounted on microscope slides almost exclusively.

1. Make sure that light is visible from base of the microscope. This indicates that light is, indeed, being transmitted to the sample.    
2. Open the path to the camera by retracting the light path selector knob all the way. VIEW
3. Use the light intensity adjustment knob to control the brightness of the image. VIEW
4. Make sure the filter turret is in position 1, indicating brightfield imaging. VIEW
5. Select a lower objective lens to find the sample and then increase the objective strength.    
6. With each change of the objective, you will need to align the condenser.    
 6.1. Close the diaphragm until you see the outline of a circle. VIEW
 6.2. Use the condenser height adjustment knob to bring the circle into focus. VIEW
 6.3. Use the centering screws to bring the circle of light to the middle of view. VIEW
 6.4. Open the diaphragm until the border of the circle just surpasses the field of view. VIEW
7. Use manual X and Y stage control knobs to move to an area of interest. VIEW
8. Use the coarse and fine focus adjustment to create an image that is in focus. VIEW
9. In the software, select the Color Balance tab, then select the One Touch icon. A box will appear in the image viewing window. Use the box to click in an area of the image that should be white, i.e. the background. VIEW
10. Manually adjust the exposure of the image by selecting the Manual Exposure Mode in the Capture tab. VIEW
11. Use the Exposure Time slider bar to produce a suitable image. VIEW
12. Once a satisfactory image is obtained, select the proper Still-Image Size. VIEW
Capture Icon13. Select the camera icon to capture the image. A new window will appear with the captured image.    
14. Once the image collection is complete, save your image as a JPEG or TIF file.    

 

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