eTraining Introduction

Specimen Preparation

Leica Ultracut UCT

Histology

JEOL JSM-6400 SEM

Hitachi S-4700 FE-SEM

Hitachi FB-2000A FIB

Microanalysis

Veeco Dim 3000 AFM

Fluorescence Microscopes

Support

Shut Down

1. Select the Stage folder and click Home. Be sure to check out any warning concerning the position of the deposition Gun.    
2. Click File > Quit from the software element you are working in and OK when prompted.    
3. Click HV to turn off the high voltage.    
4. Close the S.C. AIRLOCK VALVE from the vacuum control panel on the column console beside you.    
5. Click the Exit button to close the FB-2000A software. At the next prompt select Exit to return to the FB-2000A user login page.    
6. See Specimen Exchange in Operating Procedures under Hitachi FB-2000A FIB for instructions on removing and replacing the specimen holders. The holder should be in the FIB position. NOTE: ALWAYS REMOVE AND REPLACE HOLDERS WITH THE FIB POWER ON.    
7. After a Specimen Holder or Plug is replaced into the goniometer, turn the FIB POWER SWITCH OFF.    
8. Pick up your tools and clean the specimen preparation area. Put all specimen holders into their boxes.    
9. Swipe out of the computer system and sign the logbook, noting any problems you encountered. For any serious problems, leave Owen Mills a note on his white board.    

NOTE: YOU MUST STOP THE FIB AFTER IMAGING IS COMPLETE OR THE SPECIMEN MAY BE DAMAGED.

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